A cost-effective fluorescence detection system for pulsed laser analysis

作者：Kerns P M; Lafferty J W; Fox N A; Basu S^*

来源：Measurement, 2015, 61: 107-110.

DOI：10.1016/j.measurement.2014.10.027

• Fluorescence microscopy
• LabVIEW
• Data acquisition

A cost-effective fluorescence detection system has been developed using a National Instruments PCI-6251 data acquisition (DAQ) board that is driven by LabVIEW Signal Express. The signal is collected using a collimator and transported to the DAQ board using a fiber-coupled detector. The same detection system has been incorporated into an inverted microscope fitted with internal dichroic mirrors to provide a cost-effective alternative to commercial fluorescence microscopes. The detection system has been used to measure fluorescence intensity and generate a laser power curve that can be used for pulsed lasers. Different data analysis approaches have been compared and standard deviation and signal-to-noise ratios have been determined.

• 出版日期2015-2