AU-rich elements in the 3 '-UTR regulate the stability of the 141 amino acid isoform of parathyroid hormone-related protein mRNA

作者：Luchin Alexander I; Nadella Murali V P; Thudi Nanda K; Dirksen Wessel P; Gulati Parul; Fernandez Soledad A; Rosol Thomas J^*

来源：Molecular and Cellular Endocrinology, 2012, 364(1-2): 105-112.

DOI：10.1016/j.mce.2012.08.015

• Parathyroid hormone-related protein
• mRNA stability
• 3 '-Untranslated region
• AU-rich elements
• Tristetraproline

We demonstrated previously that parathyroid hormone-related protein (PTHrP) 1-141 mRNA is the least stable of three isoforms and is the only isoform that is stabilized by TGF-beta. In order to understand how PTHrP mRNA is stabilized by TGF-beta, we first sought to elucidate the mechanism(s) that are responsible for the instability of PTHrP isoform 1-141 mRNA. The 3'-UTR of isoform 1-141 contains four AU-rich elements (AREs), which are known to mediate mRNA degradation. We utilized a luciferase reporter system to test whether these four AREs are responsible for the short half-life of PTHrP 1-141 mRNA. Our results demonstrated that ARE elements in the 3'-UTR of PTHrP 1-141 mRNA play a significant role in regulation of the stability of the mRNA. It is known that AREs mediate their effects on mRNA stability through a number of ARE-binding proteins that recruit the exosome, a complex of exonucleases that degrades the mRNA. We identified tristetraproline (UP) as an RNA-binding protein that may be involved in ARE-mediated degradation of PTHrP 1-141 mRNA.

• 出版日期2012-11-25