An Anilinoquinazoline Derivative Inhibits Tumor Growth through Interaction with hCAP-G2, a Subunit of Condensin II

作者：Shiheido Hirokazu^*; Naito Yuhei; Kimura Hironobu; Genma Hiroaki; Takashima Hideaki; Tokunaga Mayuko; Ono Takao; Hirano Tatsuya; Du Wenlin; Yamada Taketo; Doi Nobuhide; Iijima Shiro; Hattori Yutaka; Yanagawa Hiroshi

来源：PLos One, 2012, 7(9): e44889.

DOI：10.1371/journal.pone.0044889

We screened 46 novel anilinoquinazoline derivatives for activity to inhibit proliferation of a panel of human cancer cell lines. Among them, Q15 showed potent in vitro growth-inhibitory activity towards cancer cell lines derived from colorectal cancer, lung cancer and multiple myeloma. It also showed antitumor activity towards multiple myeloma KMS34 tumor xenografts in lcr/scid mice in vivo. Unlike the known anilinoquinazoline derivative gefitinib, Q15 did not inhibit cytokine-mediated intracellular tyrosine phosphorylation. Using our mRNA display technology, we identified hCAP-G2, a subunit of condensin II complex, which is regarded as a key player in mitotic chromosome condensation, as a Q15 binding partner. Immunofluorescence study indicated that Q15 compromises normal segregation of chromosomes, and therefore might induce apoptosis. Thus, our results indicate that hCAP-G2 is a novel therapeutic target for development of drugs active against currently intractable neoplasms.

• 出版日期2012-9-13