Analysis of the in planta transcriptome expressed by the corn pathogen Pantoea stewartii subsp stewartii via RNA-Seq

作者：Packard Holly; Burke Alison Kernell; Jensen Roderick V; Stevens Ann M^*

来源：PeerJ, 2017, 5: e3237.

DOI：10.7717/peerj.3237

• Transcriptome analysis
• Pantoea stewartii
• Phytopathogen
• Corn
• RNA-Seq

Pantoea stewartii subsp. stewartii is a bacterial pytopathogen that causes Stewart's wilt disease in corn. It uses quorum sensing to regulate expression of some genes involved in virulence in a cell density-dependent manner as the bacterial population grows from small numbers at the initial infection site in the leaf apoplast to high cell numbers in the xylem where it forms a biofilm. There are also other genes important for pathogenesis not under quorum-sensing control such as a Type III secretion system. The purpose of this study was to compare gene expression during an in planta infection versus either a pre-inoculum in vitro liquid culture or an in vitro agar plate culture to identify genes specifically expressed in planta that may also be important for colonization and/or virulence. RNA was purified from each sample type to determine the transcriptorne via RNA-Seq using Illumina sequencing of cDNA. Fold gene expression changes in the in planta data set in comparison to the two in vitro grown samples were determined and la list of the most differentially expressed genes was generated to elucidate genes important for plant association. Quantitative reverse transcription PCR (qRT-PCR) was fused to validate expression patterns for a select subset of genes. Analysis off the transcriptome data via gene ontology revealed that bacterial transporters and systems important for oxidation reduction processes appear to play a critical role for P. stewartii as it colonizes and causes wilt disease in corn plants.

• 出版日期2017-4-27
• 单位美国弗吉尼亚理工大学(Virginia Tech)