A superoxide anion generator, pyrogallol induces apoptosis in As4.1 cells through the depletion of intracellular GSH content

作者：Park Woo Hyun^*; Han Yong Whan; Kim Suhn Hee; Kim Sung Zoo

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2007, 619(1-2): 81-92.

DOI：10.1016/j.mrfmmm.2007.02.004

• pyrogallol
• ROS
• cell cycle
• apoptosis
• As4.1
• ROS scavenger
• SOD
• catalase
• GSH

We investigated the involvement of ROS such as H2O2 and O-2(center dot-), and GSH in As4.1 cell death induced by pyrogallol. The intracellular H2O2 levels were decreased or increased depending on the concentration and incubation time of pyrogallol. The levels of O-2(center dot-) were significantly increased. Pyrogallol reduced the intracellular GSH content. And ROS scavengers, Tempol, Tiron, Trimetazidine and NAC could not significantly down-regulate the production of H2O2 and O-2(center dot-). However, these ROS scavengers slightly inhibited apoptosis. Interestingly, Tempol showing the recovery of GSH depletion induced by pyrogallol significantly decreased apoptosis without the significant reduction of intracellular O-2(center dot-) levels. SOD and catalase did not change the level of H2O2 but decreased the level of O-2(center dot-). The inhibition of GSH depletion by these was accompanied with the decrease of apoptosis, as evidenced by sub-GI DNA content, annexin V staining, mitochondria membrane potential (Delta psi(m)) and Western data. In addition, ROS scavengers and SOD did not alter a G2 phase accumulation of the cell cycle induced by pyrogallol. However, catalase changed the cell cycle distributions of pyrogallol-treated cells to those of pyrogallol-untreated cells. In summary, we have demonstrated that pyrogallol potently generates ROS, especially O-2(center dot-), in As4.1 JG cells, and Tempol, SOD and catalase could rescue to a lesser or greater extent cells from pyrogallol-induced apoptosis through the up-regulation of intracellular GSH content.

• 出版日期2007-6-1
• 单位河北医科大学