Application of ultrasound in preparing pathological sections to reduce processing time

作者：Chen Qian; Zou Xinye; Cheng Jianchun; Wu Junru^*

来源：Ultrasonics, 2007, 47(1-4): 78-81.

DOI：10.1016/j.ultras.2007.07.006

• ultrasound
• tissue processing
• cell membrane permeability
• pathological sections

It has been found experimentally that application of sub-mega and low megahertz ultrasound ( US) of spatial and temporal averaged intensity I-sata up to 10 W/cm(2) during the process of preparing pathological sections of the mouse tissue has shortened the processing time from 12 h ( without US) to less than half an hour ( with US). The experiment has also showed that the processing time reached the shortest for ultrasound f = 200 kHz among the frequencies of 200 kHz, 400 kHz, 600 kHz, 800 kHz and 1 MHz used in this study. It has been proposed that ultrasound inducing non-inertial cavitation enhanced the permeability of cell membrane to liquid. Thus tissue fixation and dehydration were speeded up by application of US.

• 出版日期2007-12
• 单位南京大学