Detection of All Species of the Genus Alphavirus by Reverse Transcription-PCR with Diagnostic Sensitivity

作者:Grywna Klaus; Kupfer Bernd; Panning Marcus; Drexler Jan Felix; Emmerich Petra; Drosten Christian*; Kuemmerer Beate M
来源:Journal of Clinical Microbiology, 2010, 48(9): 3386-3387.
DOI:10.1128/JCM.00317-10

摘要

Clinical arbovirus screening requires exclusion of a broad range of viruses with as few assays as possible. We present a reverse transcription-PCR (RT-PCR) for the detection of all species of the genus Alphavirus qualified for exclusion screening (limit of detection [LOD], 5 to 100 RNA copies per reaction across all Alphavirus species; detection of viremia down to ca. 10,000 copies per ml).

  • 出版日期2010-9