Dynamic in vivo imaging of small animal brain using pulsed laser diode-based photoacoustic tomography system

作者：Upputuri Paul Kumar; Pramanik Manojit^*

来源：Journal of Biomedical Optics, 2017, 22(9): 090501.

DOI：10.1117/1.JBO.22.9.090501

• photoacoustic tomography
• in vivo imaging
• brain imaging
• contrast agent
• high-speed imaging

We demonstrate dynamic in vivo imaging using a low-cost portable pulsed laser diode (PLD)-based photoacoustic tomography system. The system takes advantage of an 803-nm PLD having high-repetition rate similar to 7000 Hz combined with a fast-scanning single-element ultrasound transducer leading to a 5 s cross-sectional imaging. Cortical vasculature is imaged in scan time of 5 s with high signal-to-noise ratio similar to 48. To examine the ability for dynamic imaging, we monitored the fast uptake and clearance process of indocyanine green in the rat brain. The system will find applications to study neurofunctional activities, characterization of pharmacokinetic, and biodistribution profiles in the development process of drugs or imaging agents.

• 出版日期2017-9