Detection of autophagy based on conversions of MAP1LC3 and p62/SQSTM1

作者：Wysokinska Edyta; Kalas Wojciech^*

来源：Postepy Higieny i Medycyny Doswiadczalnej, 2013, 67: 442-448.

• MAP1LC3
• LC3
• autophagy
• fluorescence methods
• Western blotting
• p62/SQSTM1

Autophagy is a cellular process fundamental for the survival of nutrient deficiency periods and for organelle turnover. Recently much attention has been focused on autophagy as its impairment has been found in many human diseases. Unfortunately, our apparatus for study of the autophagy process is still unsatisfactory and not very well known. In this paper we would like to shed light on and discuss autophagy methods. We present the methods (fluorescence and Western blotting) based on conversions of MAP1LC3 and p62/SQSTM1 proteins, which are the most common markers of the autophagy process.

• 出版日期2013-5-20