Tomato yellow leaf curl China virus: monopartite genome organization and agroinfection of plants

作者：Yin QY; Yang HY; Gong QH; Wang HY; Liu YL; Hong YG; Tien P^*

来源：Virus Research, 2001, 81(1-2): 69-76.

DOI：10.1016/S0168-1702(01)00363-X

• Begomovirus
• tomato yellow leaf curl virus
• monopartite genome
• agroinfection

The complete DNA sequence (2734 nucleotides) of the monopartite genome of tomato yellow leaf curl China virus (TYLCCNV), a begomovirus transmitted by the whitefly Bemisia tabaci, was determined. The circular genomic DNA contains six open reading frames (ORFs) encoding proteins of molecular weights > 10 kDa, of which two (V1 and V2) are located on the virion-sense strand and four (C1, C2, C3 and C4) on the complementary-sense strand. The ORFs are comparable to those of other whitefly-transmitted begomoviruses with a monopartite genome and to those encoded by DNA-A of bipartite begomoviruses. Sequence comparisons with other geminiviruses showed that TYLCCNV belongs to Begomovirus from the Old World. No putative DNA-B genome was found. Nicotiana species and tomato plants agroinoculated with the TYLCCNV monopartite genome developed typical yellowing and leaf-curling symptoms. The cloned molecule carried all the information needed for virus replication and systemic infection of plants.

• 出版日期2001-12-4
• 单位中国科学院微生物研究所