A Novel In Vivo Model for Evaluating Functional Restoration of a Tissue-Engineered Salivary Gland

作者：Pradhan Bhatt Swati; Harrington Daniel A; Duncan Randall L; Farach Carson Mary C; Jia Xinqiao; Witt Robert L^*

来源：Laryngoscope, 2014, 124(2): 456-461.

DOI：10.1002/lary.24297

• Bioartificial organ
• three-dimensional cell culture
• salivary gland
• tissue engineering
• regeneration
• progenitor cells
• hyaluronic acid-based hydrogels
• hyaluronic acid receptors

Objectives/HypothesisTo create a novel model for development of a tissue-engineered salivary gland from human salivary gland cells that retains progenitor cell markers useful for treatment of radiation-induced xerostomia.
Study DesignA three-dimensional (3D) hyaluronic acid (HA)-based hydrogel scaffold was used to encapsulate primary human salivary gland cells and to obtain organized acini-like spheroids. Hydrogels were implanted into rat models, and cell viability and receptor expression were evaluated.
MethodsA parotid gland surgical resection model for xenografting was developed. Salivary cells loaded in HA hydrogels formed spheroids and in vitro were implanted in the three-fourths resected parotid bed of athymic rats. Implants were removed after 1 week and analyzed for spheroid viability and phenotype retention.
ResultsSpheroids in 3D stained positive for HA receptors CD168/RHAMM and CD44, which is also a progenitor cell marker. The parotid gland three-fourths resection model was well-tolerated by rodent hosts, and the salivary cell/hydrogel scaffolds were adherent to the remaining parotid gland, with no obvious signs of inflammation. A majority of human cells in the extracted hydrogels demonstrated robust expression of CD44.
ConclusionsA 3D HA-based hydrogel scaffold that supported long-term culture of salivary gland cells into organized spheroids was established. An in vivo salivary gland resection model was developed that allowed for integration of the 3D HA hydrogel scaffold with the existing glandular parenchyma. The expression of CD44 among salivary cultures may partially explain their regenerative potential, and the expression of CD168/RHAMM along with CD44 may aid the development of these 3D spheroids into regenerated salivary glands
Level of EvidenceNA Laryngoscope, 124:456-461, 2014

• 出版日期2014-2