A New Source of (R)-Limonene and Rotundifolone from Leaves of Lippia pedunculosa (Verbenaceae) and their Trypanocidal Properties

作者：Alencar Menezes Leociley Rocha; Santos Nilmara Nunes; Meira Cassio Santana; Ferreira dos Santos Jamyle Andrade; Guimaraes Elisalva Teixeira; Pereira Soares Milena Botelho; Nepel Angelita; Barison Andersson; Costa Emmanoel Vilaca^*

来源：Natural Product Communications, 2014, 9(6): 737-739.

DOI：10.1177/1934578x1400900601

• Lippia pedunculosa
• Essential oil
• Trypanocidal activity
• Monoterpenes
• Limonene
• Rotundifolone

Investigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves and identified by spectrometric analysis. LPOE and isolated compounds were evaluated for their trypanocidal activity against epimastigote and trypomastigote forms of Trypanosoma cruzi. Significant results with IC50 values lower than 34.0 mu g.mL(-1) were observed against these forms of T. cruzi for LPOE and isolated compounds. Rotundifolone was the most active compound with an IC50 lower than 10.0 mu g.mL(-1) for both forms of T. cruzi. The effects of LPOE and isolated compounds were also evaluated in cultures of macrophages infected with T. cruzi. Treatment with (R)-limonene and rotundifolone caused a moderate reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites at concentrations non-toxic to macrophages.

• 出版日期2014-6