科研之友
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摘要
Based on the two-dimensional data produced by gas chromatography-mass spectrometry hyphenated instrument, a new chemometrics method called moving subwindow searching(MSS) using pure spectra as standard to search a certain retention time range in another chromatograms then obtain the eluted range of the target compound, in which the middle point is thought to be its, retention time and the retention indices could be obtained, was proposed as an complementary technique to gas chromatography-mass spectrometry(GC-MS) for qualitative analysis. Thus, at a temperature program of 80 degrees C to 290 degrees C at 4 degrees C/min, combined GC-MS with temperature-programmed retention indices(I-T) of the identified compounds at two different polarity fused silica columns (HP-5MS and DB-35MS) obtained through MSS, essential oils from buds and flowers of Syringa oblata Lindl grown in Heilongjiang province were identified unambiguously. As a result, the qualitative results obtained from GC-MS with the help of chemometrics and IT at two columns with different polarity were more quickly and reliable.
