Additive effect of mesenchymal stem cells and defibrotide in an arterial rat thrombosis model

作者：Dilli Dilek^*; Kilic Emine; Yumusak Nihat; Beken Serdar; Cetinkaya Duygu Uckan; Karabulut Ramazan; Zenciroglu Aysegul

来源：Archivos Argentinos de Pediatria, 2017, 115(3): 249-256.

DOI：10.5546/aap.2017.eng.249

• Thrombosis
• mesenchymal stem cell
• defibrotide
• rats

Background/aim. In this study, we aimed to investigate the additive effect of mesenchymal stem cells (MSC) and defibrotide (DFT) in a rat model of femoral arterial thrombosis.
Methods. Thirty Sprague Dawley rats were included. An arterial thrombosis model by ferric chloride (FeCl3) was developed in the left femoral artery. The rats were equally assigned to 5 groups: Group 1-Sham-operated (without arterial injury); Group 2-Phosphate buffered saline (PBS) injected; Group 3-MSC; Group 4-DFT; Group 5-MSC + DFT. All had two intraperitoneal injections of 0.5 ml: the 1st injection was 4 h after the procedure and the 2nd one 48 h after the 1st injection. The rats were sacrificed 7 days after the 2nd injection.
Results. Although the use of human bone marrow-derived (hBM) hBM-MSC or DFT alone enabled partial resolution of the thrombus, combining them resulted in near-complete resolution. Neovascularization was two-fold better in hBM-MSC + DFT treated rats (11.6 +/- 2.4 channels) compared with the hBM-MSC (3.8 +/- 2.7 channels) and DFT groups (5.5 +/- 1.8 channels) (P < 0.0001 and P= 0.002, respectively).
Conclusion. The combined use of hBM-MSC and DFT in a rat model of arterial thrombosis showed additive effect resulting in near-complete resolution of the thrombus.

• 出版日期2017-6