iRoot FM exerts an antibacterial effect on Porphyromonas endodontalis and improves the properties of stem cells from the apical papilla

作者：Bi, J.; Liu, Y.; Liu, X. M.; Jiang, L. M.; Chen, X.^*

来源：International Endodontic Journal, 2018, 51(10): 1139-1148.

DOI：10.1111/iej.12923

• antibacterial activity
• iRoot FM
• osteo-
• odontogenic differentiation
• Porphyromonas endodontalis
• proliferation
• stem cells from apical papilla

AimTo investigate the antibacterial activity of a novel intracanal medicament, iRoot FM, against Porphyromonas endodontalis and its effects on the proliferation and osteo-/odontogenic differentiation of stem cells from apical papilla (SCAP). @@@ MethodologyThe agar diffusion test was used to compare the antimicrobial efficacy of iRoot FM with two traditional intracanal medicaments, calcium hydroxide [Ca(OH)(2)] and triple antibiotic paste (TAP). The CCK-8 assay was used to assess the proliferation rate of SCAP when exposed to the three intracanal medicaments. The expression levels of ALP and DMP-1 and the capacity to form mineralized nodules were used to evaluate the osteo-/odontogenic differentiation of SCAP, as assessed by real-time PCR, Western blotting and alizarin red S staining. Data were statistically analysed with one-way analysis of variance (anova), and comparisons between each of two groups were analysed by the least significance difference method. P values less than 0.05 were considered statistically significant. @@@ ResultsThe zone of inhibition against P.endodontalis produced by iRoot FM was 20.744.35mm, whilst the zones of inhibition of Ca(OH)(2) and TAP were 24.89 +/- 3.84mm and 34.51 +/- 1.20mm. The antibacterial capacity of iRoot FM was similar to that of Ca(OH)(2) (P>0.05). SCAP, cultured in conditioned medium with iRoot FM, was associated with greater proliferation and osteo-/odontogenic differentiation capacity than those cultured in conditioned medium with Ca(OH)(2) and TAP (P<0.05). Moreover, iRoot FM had no negative effects on the proliferation rate of SCAP. @@@ ConclusionsiRoot FM exhibited excellent antibacterial activity against P.endodontalis and could improve the proliferation and differentiation of SCAP. The findings provide evidence that iRoot FM has potential as an intracanal medicament for endodontic procedures in immature permanent teeth.

• 出版日期2018-10
• 单位中国医科大学