A compact holographic optical tweezers instrument

作者：Gibson G M^*; Bowman R W; Linnenberger A; Dienerowitz M; Phillips D B; Carberry D M; Miles M J; Padgett M J

来源：Review of Scientific Instruments, 2012, 83(11): 113107.

DOI：10.1063/1.4768303

Holographic optical tweezers have found many applications including the construction of complex micron-scale 3D structures and the control of tools and probes for position, force, and viscosity measurement. We have developed a compact, stable, holographic optical tweezers instrument which can be easily transported and is compatible with a wide range of microscopy techniques, making it a valuable tool for collaborative research. The instrument measures approximately 30x30x35 cm and is designed around a custom inverted microscope, incorporating a fibre laser operating at 1070 nm. We designed the control software to be easily accessible for the non-specialist, and have further improved its ease of use with a multi-touch iPad interface. A high-speed camera allows multiple trapped objects to be tracked simultaneously. We demonstrate that the compact instrument is stable to 0.5 nm for a 10 s measurement time by plotting the Allan variance of the measured position of a trapped 2 mu m silica bead. We also present a range of objects that have been successfully manipulated.

• 出版日期2012-11