A simple and sensitive electrochemical immunosensor based on thiol aromatic aldehyde as a substrate for the antibody immobilization

作者：Shen, Youming^*; Zhang, Youyu; Liu, Meiling; Liu, Xiaoyin; Guo, Huan; Zhang, Xiangyang; Zhang, Chunxiang; Li, Haitao; Yao, Shouzhuo

来源：Talanta, 2015, 141: 288-292.

DOI：10.1016/j.talanta.2015.04.004

• Synthesis
• Thiol aromatic aldehyde
• Antibody immobilization
• Electrochemical immunosensor
• Human IgG

In this work, a novel sulfur-containing compound with aldehyde groups was synthesized, which was used to immobilize antibodies through the covalent bonding of the aldehyde groups with amino groups, in which no additional chemical cross-linker is required. Human IgG was used as a model analyte to fabricate the electrochemical immunosensor. Using the proposed immunosensor, IgG was detected within the range from 0.01 to 25 ng mL(-1) with a detection limit of 0.003 ng mL(-1) obtained by 3 S/N. The simple electrochemical immunosensor had a good specificity, stability and reproducibility.

• 出版日期2015-8-15
• 单位湖南师范大学; 湖南文理学院; 湖南农业大学