Amperometric and Photometric Responses of in Situ Coupled Glucose Oxidase-Poly (Propylene Imine) Dendrimer Based Glucose Biosensor

作者：Shukla Sudheesh K^*; Mishra Ajay K; Mamba Bhekie B; Arotiba Omotayo A

来源：International Journal of Electrochemical Science, 2013, 8(10): 11711-11722.

DOI：10.1016/s1452-3981(23)13216-0

• glucose oxidase
• poly (propylene imine) dendrimer
• biosensor
• glucose amperometric
• photometric

In this work, poly (propylene imine) dendrimer (PPI) was coupled to glucose oxidase enzyme (GOx) in situ and used as a bioreceptor in a glucose biosensor. The coupling was accomplished by the use of 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) linker. Ultraviolet visible spectroscopy, Fourier transform infra red spectroscopy and scanning electron microscopy were used to confirm the coupling reaction. A biosensor was then prepared by drop coating the GOx-PPI onto a glassy carbon electrode and then used for the amperometric detection of glucose. The bio-recognition of glucose by the GOx-PPI was observed with a detection limit of 0.1 mM. Photometric responses showed good enzyme stability and activity with a Michaelis-Menten constant of 0.127 mM.

• 出版日期2013-10