科研之友
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摘要
Background: Ankylosing spondylitis (AS) involves inflammation at the sacroiliac joint and spine attachment site. This study aimed to observe the ratio and function of peripheral regulatory V delta 1 T cells in AS patients to investigate their roles in AS pathogenesis. Material/Methods: Peripheral blood mononuclear cells (PBMC) were separated by density-gradient centrifugation from AS patients and healthy controls. Flow cytometry was used to determine the ratio between V delta 1 and CD4 T cells of PBMC in AS patients and controls. Flow cytometry sorting (FCS) was used to obtain V delta 1 and naive CD4 T cells with purity higher than 90%. CFSE staining method was used to detect the effect of V delta 1 T cells on proliferation of naive CD4 T cells. The effect of V delta 1 T cells on secretion of IFN-gamma from naive CD4 T cells and the ability to secrete IL-10 from V delta 1 T cells were determined by flow cytometry. Results: AS patients had significantly lower V delta 1 T cell ratio in PBMC compared to controls (p<0.05), but their CD4 T cell ratio was significantly elevated (p<0.05). Functional assay showed suppression of naive CD4 T cell proliferation and IFN-gamma secretion by peripheral V delta 1 T cells in AS patients (p<0.01). AS patients also had lower IL-10 secreting level from peripheral derived V delta 1 T cells (p<0.01). Conclusions: The immune suppression of peripheral V delta 1 T cell in AS patient increases the ratio of peripheral CD4 T cells and IFN-gamma level, leading to AS pathogenesis. This immune suppression is mainly due to suppressed IL-10 secretion.
