科研之友
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摘要
Background and Objective:The genus Tribulus of the Zygophyllaceae family comprises several species which grow as shrubs or herbs in subtropical areas around the world. Phytochemical and biological studies of the different successive extracts of the aerial parts of Tribulus terrestris L (Family Zygophyllaceae), growing in Egypt as well as the isolation and identification of the biologically active natural fractions or compounds were carried out. Methodology: The volatile constituents were prepared and analyzed by GC/MS. The lipoidal matter was prepared and analyzed by GLC. The carbohydrate and the amino acid contents were prepared and analyzed by HPLC. Saponin fraction was prepared. The activities of polar, non-polar extracts and saponin fraction as aphrodisiac, antimicrobial, cytotoxic and antioxidant were evaluated. Results:GC/MS of volatile constituents revealed the presence of thirty two compound s. The major compound was methyl linolenate (18.56%). The major fatty acid in the lipoidal matter is heptadecanoic acid (33.56%). HPLC analysis of carbohydrate and amino acid revealed the presence of inulin (5.61%) and glutamic acid (2.85%), respectively. Saponin yield was 7.38%. The results of the biological investigation of the polar and non-polar extracts revealed their aphrodisiac, antimicrobial, cytotoxic and antioxidant activities with different percentages. Phytochemical examination of the aqueous methanolic biologically active extract by HPLC revealed the presence of phenolic and flavonoid contents (11.16 and 6.076%) respectively as well as saponin. Isolation and identification of rutin, quercetin and diosgenin were carried out using several chromatographic and spectroscopic analysis. Conclusion: The herb under investigation revealed potent and remarkable biological activities due to the presence of different active constituents.
- 出版日期2018
