Analysis of rare flavonoid C-glycosides in Celtis australis L. by micellar electrokinetic chromatography

作者：Zehrmann Nina; Zidorn Christian; Ganzera Markus^*

来源：Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(5): 1165-1168.

DOI：10.1016/j.jpba.2009.11.028

• Celtis australis
• Flavonoid
• C-glycoside
• MEKC

This manuscript reports on the first analytical procedure for the determination of flavonoids in Celtis australis. The capillary electrophoretic separation of 8 compounds, most of them flavone C-glycosides, was possible using a borax buffer with pH 9.0, which contained 25 mM SDS as detergent and 7.5% of n-butanol as organic modifier. Method validation revealed that the developed assay is repeatable (sigma(rel) <= 4.0%), precise (inter-day sigma(rel) <= 6.7%, intra-day sigma(rel) <= 3.9%), accurate (recovery rates from 96.8 to 102.3%), sensitive (LOD: 2.2-1.6 mu g/ml) and linear (R(2) >= 0.9996) within the tested concentration range. The quantitative analysis of several C australis samples showed that isovitexin is the most abundant representative (0.06-0.09%), at a rather uniform content of total flavonoids of approx. 0.3% in all specimens.

• 出版日期2010-4-6