Downregulation of Nuclear Respiratory Factor-1 Contributes to Mitochondrial Events Induced by Benzo(a)pyrene

作者：Zhang Lijuan; Bao Yan; Liu Yimin; Li Jue^*

来源：Environmental Toxicology, 2014, 29(7): 780-787.

DOI：10.1002/tox.21805

• benzo(a)pyrene
• nuclear respiratory factor 1
• mitochondrial transcription factor A
• mitochondrial events

Environmental carcinogen benzo(a) pyrene (BaP) has been shown to be a genotoxicant that affects both nuclear DNA (nDNA) and mitochondrial DNA (mtDNA). Nuclear respiratory factor 1 (NRF-1) is a transcriptional activator of nuclear genes that encode a range of mitochondrial proteins including mitochondrial transcription factor A (mtTFA). However, the role of NRF-1 in BaP-induced mitochondrial event is not clear. We investigated the change of NRF-1 and mtTFA in human bronchial epithelial cells (16HBE) elicited by BaP. The results indicated that BaP induced cell apoptosis, total mitochondrial enzymes activities and ATP levels decrease in dose-and time-dependent manners, respectively. The transcription and protein levels of NRF-1 and mtTFA decreased at 48 h after 16 mu M BaP treatment. Our results indicated downregulation of NRF-1 and mtTFA is involved in BaP-induced mitochondrial events.

• 出版日期2014-5
• 单位同济大学