Detection of IL-17A-producing peripheral blood monocytes in Langerhans cell histiocytosis patients

作者：Lourda Magda^*; Olsson Akefeldt Selma; Gavhed Desiree; Bjornfot Sofia; Clausen Niels; Hjalmars Ulf; Sabel Magnus; Tazi Abdellatif; Arico Maurizio; Delprat Christine; Henter Jan Inge; Svensson Mattias

来源：Clinical Immunology, 2014, 153(1): 112-122.

DOI：10.1016/j.clim.2014.04.004

• Monocytes
• Langerhans cell histiocytosis
• Interleukin-17A
• Retinoic acid orphan receptor gamma t
• Retinoic acid orphan receptor C

Langerhans cell histiocytosis (LCH) is a rare disease of unknown cause with manifestations ranging from isolated granulomatous lesions to life-threatening multi-system organ involvement. This disorder is further characterized by infiltration of immune cells in affected tissues and an association with interleukin (IL)-17A has been reported. Here, we investigated the presence of IL-17A-producing cells among peripheral blood mononuclear cells isolated from LCH patients and observed a high percentage of IL-17A(+) monocytes in peripheral blood of LCH patients compared to controls. The IL-17A(+) monocytes were also positive for the transcription factor retinoic acid orphan receptor (ROR) gamma t and showed increased mRNA levels for both IL-17A and ROR gamma t. Notably, IL-17A was produced by all monocyte subsets and the expression level was positively associated with LCH disease activity. These data support a role for monocytes in the pathogenesis of LCH. Future therapeutic approaches may consider identification of patients who may benefit from IL-17A-targeted interventions.

• 出版日期2014-7