Delaying the Onset of Resistance Formation: Effect of Manipulating Dose, Wavelength, and Rate of Energy Delivery of 405-, 464-, and 850-Nanometer Light for Staphylococcus aureus

作者：Guffey J Stephen^*; Payne William; Martin Kyle; Dodson Carly

来源：Wounds, 2014, 26(4): 95-100.

• blue light
• Staphylococcus aureus
• resistance formation

Objective. To determine whether manipulation of dose, wavelength, and rate of energy delivery could delay the onset of previously demonstrated Staphylococcus aureus resistance to blue light. Materials and Methods. The organism was treated in vitro with 405 nm, 464 nm, and combined 464 nm and with 850 nm light emitted from a supraluminous diode (SLD) array. Doses of 9 J/cm(2) and 30 J/cm(2) were used. Rates of energy delivery were also varied from 10 mW/cm(2) to 125 mW/cm(2). Seven stages were employed to test for resistance formation. Colony counts were performed and compared to untreated controls using Student t tests and one-way ANOVA with Tukey post hoc analysis. Results. A best dose, wavelength, and rate of delivery combination was determined at each stage and it did produce a significant kill rate (P <= 0.05) at each stage. Analysis of variance demonstrated that no loss of effectiveness (formation of resistance) occurred over the 7 stages. Conclusions. Appropriate combinations of dose, wavelength, and rate of energy delivery can delay resistance formation to light as a bactericidal agent for S. aureus.

• 出版日期2014-4