An ultra-sensitive biophysical risk assessment of light effect on skin cells

作者：Bennet Devasier; Viswanath Buddolla; Kim Sanghyo^*; An Jeong Ho^*

来源：Oncotarget, 2017, 8(29): 47861-47875.

DOI：10.18632/oncotarget.18136

• biophysics of organization
• biomechanical changes
• color light radiation
• extracellular matrix degeneration
• AFM and ECIS based analysis

The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultrastructural changes, damages, and ECM impair function were evaluated by up/downregulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. Overall, the obtained results identify a new insight that may contribute to premature aging, and causes it to look aged in younger people. Moreover, these results advance our understanding of the different color light-induced degenerative process and help the development of new therapeutic strategies.

• 出版日期2017-7-18