A quantitative theory model of a photobleaching mechanism

作者：Chen TS; Zeng SQ; Zhou W; Luo QM^*

来源：Chinese Physics Letters, 2003, 20(11): 1940-1943.

DOI：10.3321/j.issn:0256-307X.2003.11.010

A photobleaching model: D-P (dye-photon interaction) and D-O (Dye-oxygen oxidative reaction) photobleaching theory, is proposed. The quantitative power dependences of photobleaching rates with both one- and two-photon excitations (I PE and TPE) are obtained. This photobleaching model can be used to elucidate our and other experimental results commendably. Experimental studies of the photobleaching rates for rhodamine B with TPE under unsaturation conditions reveals that the power dependences of photobleaching rates increase with the increasing dye concentration, and that the photobleaching rate of a single molecule increases in the second power of the excitation intensity, which is different from the high-order (> 3) nonlinear dependence of ensemble molecules.

• 出版日期2003-11
• 单位华中科技大学