Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering

作者：Puppi Dario; Mota Carlos; Gazzarri Matteo; Dinucci Dinuccio; Gloria Antonio; Myrzabekova Mairam; Ambrosio Luigi; Chiellini Federica^*

来源：Biomedical Microdevices, 2012, 14(6): 1115-1127.

DOI：10.1007/s10544-012-9677-0

• Tissue engineering
• Scaffolds
• Wet-spinning
• Additive manufacturing
• Polycaprolactone

An Additive Manufacturing technique for the fabrication of three-dimensional polymeric scaffolds, based on wet-spinning of poly(epsilon-caprolactone) (PCL) or PCL/hydroxyapatite (HA) solutions, was developed. The processing conditions to fabricate scaffolds with a layer-by-layer approach were optimized by studying their influence on fibres morphology and alignment. Two different scaffold architectures were designed and fabricated by tuning inter-fibre distance and fibres staggering. The developed scaffolds showed good reproducibility of the internal architecture characterized by highly porous, aligned fibres with an average diameter in the range 200-250 mu m. Mechanical characterization showed that the architecture and HA loading influenced the scaffold compressive modulus and strength. Cell culture experiments employing MC3T3-E1 preosteoblast cell line showed good cell adhesion, proliferation, alkaline phosphatase activity and bone mineralization on the developed scaffolds.

• 出版日期2012-12