A Theoretical Study of Bi-Layer Osteoblast Cell Electroporation in a Micro Channel at Radio Frequency

作者：Sarkar S^*; Mahapatra R; Das S

来源：Journal of Biomaterials and Tissue Engineering, 2013, 3(6): 605-623.

DOI：10.1166/jbt.2013.1123

• Outer-Inner Membrane
• Micro Channel
• Electroporation
• Electropermeabilization
• Osteoblast Cell
• Bone Cancer Cell

Electroporation of a bi-layer osteoblast cell in a micro channel at radio frequency is studied. The effects of electrical pulse (e.g., duration, intensity and interval of the electric pulse), electrode configuration (width, inter electrode gap, shape and constrictive material of electrode), micro channel dimension (width, Height and resistances) and property of suspension media (conductivity, flow rate) on cell outer and inner membrane permeabilization in osteoblast cell, were investigated. The electrodes were assumed to be embedded in the walls of the micro channel. The cell was suspended between these two electrodes. It is observed that the efficient electroporation in outer layer is conducted with micro scale pulse duration and in inner layer with peco scale pulse duration although pulse interval is micro scale in both cases. This information supports window effects in between outer and inner layer. It is also exposed that the nature of membrane potential curves of outer and inner membrane are opposites which gives the idea about different dielectric property of the both layers. We also find out that the transmembrane potential in both layers distribution deviates from the sinusoidal behaviour and the induced transnnembrane potential also concentrates around the poles of the cell membrane. During cell membrane permeabilization, the biggest nanopores are initially created at the poles and then the nanopore population expands toward the equator. On the other hand it is found that the location of pores are same in both layers but size of the pores are different. The inner membrane pore size is higher than outer membrane pore due to the different elastic property. The radius of the created nanopores are influenced by electric pulse, electrode and micro fluidic channel specification but in all cases the biggest nanopore is created at pole (theta = 90) which is independent of above specification. As the cells are in contact together tightly so we also consider the neighbour cells to make the result more important and realistic. All the information given in this article might provide a new light on drug delivery system in bone cell.

• 出版日期2013-12