Flavanone exhibits PPAR gamma ligand activity and enhances differentiation of 3T3-L1 adipocytes

作者：Saito Takeshi; Abe Daigo; Sekiya Keizo^*

来源：Biochemical and Biophysical Research Communications, 2009, 380(2): 281-285.

DOI：10.1016/j.bbrc.2009.01.058

• 3T3-L1
• Adipocyte
• Cyclin
• ERK
• Flavanone
• PPAR gamma

Flavanones are class of polyphenolic compounds, some of which are found in foods and provide health benefits. In this study, we show that flavanone significantly enhances differentiation of 3T3-L1 preadipocytes. During adipogenesis, flavanone enhanced expression of genes and accumulation of proteins that are involved in adipocyte function. Some reports have indicated that flavanone inhibits proliferation of mammalian cells, and down-regulates expression of growth-related proteins. Such proteins include phosphorylated ERK1/2, cyclins, and Cdks that are important for an early event in adipogenesis, mitotic clonal expansion (MCE). We demonstrated that flavanone did not inhibit MCE or expression of MCE-related proteins, except for a modest inhibition of cyclin D1 expression. Using luciferase reporter assays, we found that flavanone acted as a peroxisome proliferator-activated receptor gamma (PPAR gamma) ligand in a close-dependent manner. Together, our results suggest that flavanone enhances adipogenesis, at least in part, through its PPAR gamma ligand activity.

• 出版日期2009-3-6