Directly suspended droplet microextraction in combination with microvolume UV-vis spectrophotometry for determination of phosphate

作者：Pena Pereira Francisco; Cabaleiro Noelia; de la Calle Inmaculada; Costas Marta; Gil Sandra; Lavilla Isela; Bendicho Carlos^*

来源：Talanta, 2011, 85(2): 1100-1104.

DOI：10.1016/j.talanta.2011.05.032

• Directly suspended droplet microextraction
• Microvolume spectrophotometry
• Phosphate
• Dissolved reactive phosphorus
• Preconcentration

A miniaturized methodology for the determination of phosphate in waters has been developed by combining directly suspended droplet microextraction (DSDME) with microvolume spectrophotometry. The method is based on the extraction of the ion pair formed between 12-molybdophosphate and malachite green onto a microdrop of methyl isobutyl ketone and subsequent spectrophotometric determination with no dilution. An enrichment factor of 325 was obtained after 7.5 min of microextraction. The detection limit was 6.1 nM phosphate and the repeatability, expressed as relative standard deviation, was 2.7% (n = 6). The method was successfully applied to the determination of dissolved reactive phosphorus in different freshwater samples.

• 出版日期2011-8-15