Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures

作者：Boulange Lecomte Celine; Rocher Beatrice; Cailleaud Kevin; Cosette Pascal; Legrand Elena; Devreker David; Budzinski Helene; Souissi Sami; Forget Leray Joelle^*

来源：Environmental Toxicology and Chemistry, 2016, 35(7): 1860-1871.

DOI：10.1002/etc.3343

• 2D electrophoresis
• Biomarkers
• Calanoid
• Estuary
• Organic contaminants

Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500ngL(-1); alkylphenol mixture, 1000ngL(-1)). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment. Environ Toxicol Chem 2016;35:1860-1871.

• 出版日期2016-7