Phytochemical analysis of Bacopa monnieri (L.) Wettst. and their anti-fungal activities

作者：Jain Paras; Sharma H P; Basri Fauziya; Priya Kumari; Singh Pallavi

来源：Indian Journal of Traditional Knowledge, 2017, 16(2): 310-318.

• Bacopa monnieri (L.) Wettst.
• Antoxidant activity
• Phytochemicals estimations
• GC-MS analysis

Realization of hazardous effects of synthetic fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, designed to investigate the anti-fungal activities of Bacopa monnieri (L.) Wettst and understand its possible mechanism. To estimate the anti-fungal activity, food poisoning method was applied. Extract of Bacopa effectively controlled the growth of chosen fungi as evidenced by the IC50 value- Fusarium oxysporum (31.25 mu g/ml), Sclerotium rolfsii (6.25 mu g/ml), Alternaria sp (28.75 mu g/ml), Rhizoctonia solani (18.75 mu g/ml). Phytochemical screening revealed the presence of Tannin, Phlobatannin, Saponin, Steroid, Flavonoid, Cardiac Glycoside, Phenol, Carbohydrate and Alkaloid. However, Anthraquinone and Terpenoid were absent. Quantitative analysis revealed the presence of 12.5 mg, 110 mg and 1.5 mg of tannin, alkaloid and saponin, respectively per gin dry matter of the plant extract, while phenol was estimated to be 24.75 mg GAE/gm methanolic extract. GC-MS analysis showed the presence of 35 compounds in the methanolic extract of whole plant of Bacopa monnieri. The Bacopa monnieri showed free radicals scavenging activity by DPPH method (94 % inhibition at 1000 mu g/ml), CUPRAC assay (0.22622 TE mM at 1000 mu g/ml), anthocyanin content (1.436 CGE mg/ml), flavonoid content (29.666 GAE/gm). In, conclusion, the plant extracts of Bacopa monnieri contains phytochemicals, which shows antifungal activities.

• 出版日期2017-4