Direct Reduction of Antigen Receptor Expression in Polyclonal B Cell Populations Developing In Vivo Results in Light Chain Receptor Editing

作者：Shen Shixue; Manser Tim^*

来源：The Journal of Immunology, 2012, 188(1): 47-56.

DOI：10.4049/jimmunol.1102109

Secondary Ab V region gene segment rearrangement, termed receptor editing, is a major mechanism contributing to B lymphocyte self-tolerance. However, the parameters that determine whether a B cell undergoes editing are a current subject of debate. We tested the role that the level of BCR expression plays in the regulation of receptor editing in a polyclonal population of B cells differentiating in vivo. Expression of a short hairpin RNA for kappa L chain RNA in B cells resulted in reduction in levels of this RNA and surface BCRs. Strikingly, fully mature and functional B cells that developed in vivo and efficiently expressed the short hairpin RNA predominantly expressed BCRs containing lambda light chains. This shift in L chain repertoire was accompanied by inhibition of development, increased Rag gene expression, and increased lambda V gene segment-cleavage events at the immature B cell stage. These data demonstrated that reducing the translation of BCRs that are members of the natural repertoire at the immature B cell stage is sufficient to promote editing. The Journal of Immunology, 2012, 188: 47-56.

• 出版日期2012-1-1