APPLICATION OF IMMUNOMAGNETIC PARTICLES TO ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR IMPROVEMENT OF DETECTION SENSITIVITY OF HCG

作者：Kuo, Hsiao-Ting; Yeh, Jay Z.; Wu, Po-Hua; Jiang, Chii-Ming; Wu, Ming-Chang^*

来源：Journal of Immunoassay and Immunochemistry, 2012, 33(4): 377-387.

DOI：10.1080/15321819.2012.655820

• antibody immunomagnetic particle
• enzyme-linked immunoassay
• human chorionic gonadotropin
• magnetic carrier concentration technology
• pregnancy test
• superparamagnetic particle

This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG alpha antibody to superparamagnetic particle (SPIO-anti hCG alpha antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.

• 出版日期2012
• 单位西北大学