Noninvasive In Vivo Imaging to Evaluate Immune Responses and Antimicrobial Therapy against Staphylococcus aureus and USA300 MRSA Skin Infections

作者：Cho John S; Zussman Jamie; Donegan Niles P; Ramos Romela Irene; Garcia Nairy C; Uslan Daniel Z; Iwakura Yoichiro; Simon Scott I; Cheung Ambrose L; Modlin Robert L; Kim Jenny; Miller Lloyd S^*

来源：Journal of Investigative Dermatology, 2011, 131(4): 907-915.

DOI：10.1038/jid.2010.417

Staphylococcus aureus skin infections represent a significant public health threat because of the emergence of antibiotic-resistant strains such as methicillin-resistant S. aureus (MRSA). As greater understanding of protective immune responses and more effective antimicrobial therapies are needed, a S. aureus skin wound infection model was developed in which full-thickness scalpel cuts on the backs of mice were infected with a bioluminescent S. aureus (methicillin sensitive) or USA300 community-acquired MRSA strain and in vivo imaging was used to noninvasively monitor the bacterial burden. In addition, the infection-induced inflammatory response was quantified using in vivo fluorescence imaging of LysEGFP mice. Using this model, we found that both IL-1 alpha and IL-1 beta contributed to host defense during a wound infection, whereas IL-1 beta was more critical during an intradermal S. aureus infection. Furthermore, treatment of a USA300 MRSA skin infection with retapamulin ointment resulted in up to 85-fold reduction in bacterial burden and a 53% decrease in infection-induced inflammation. In contrast, mupirocin ointment had minimal clinical activity against this USA300 strain, resulting in only a 2-fold reduction in bacterial burden. Taken together, this S. aureus wound infection model provides a valuable preclinical screening method to investigate cutaneous immune responses and the efficacy of topical antimicrobial therapies. Journal of Investigative Dermatology (2011) 131, 907-915; doi:10.1038/jid.2010.417; published online 30 December 2010

• 出版日期2011-4
• 单位河北医科大学