科研之友
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摘要
A growth trial was conducted to estimate the optimum requirement of dietary available phosphorus (P) for Chinese sucker juveniles. Triplicate groups of juveniles Chinese sucker (initial mean weight: 1.77 +/- 0.02 g, mean +/- SD) were fed diets containing graded levels (3.1, 5.3, 7.5, 9.6 and 11.8 g kg(-1)) of available phosphorus. The basal diet (diet 1), containing 3.1 g kg(-1) available P, was supplemented with graded levels of monocalcium phosphate to formulate four experimental diets. The fish were fed twice daily (08: 00 and 17: 00 h) to satiation for 8 weeks. During the experimental period, the water temperature fluctuated from 27.5 to 30.5 degrees C and dissolved oxygen was more than 6 mg L-1. The specific growth rate, protein efficiency ratio were all significantly increased by dietary available phosphorus up to 7.5 g kg(-1) (P < 0.05) and then levelled off beyond this level. Feed conversion ratio significantly decreased with dietary available phosphorus level up to 7.5 g kg(-1) (P < 0.05). Dietary treatments did not significantly affect feed intake (P > 0.05). Efficiency of phosphorus (P) utilization significantly decreased with dietary available phosphorus level (P < 0.05). Body composition analysis showed that the whole-body lipid, ash, calcium, phosphorus, magnesium (Mg), zinc (Zn) and copper (Cu) contents were all significantly affected by dietary available P concentration (P < 0.05); however, no significance were found for manganese (Mn) concentration and calcium/phosphorus (Ca/P) ratios in whole-body among all the treatments (P > 0.05). Dietary phosphorus levels also significantly affected the mineralization of vertebrae and scale (P < 0.05), and Ca/P ratios in scale were not influenced by dietary P supplementation, while vertebrae Ca/P ratio decreased with dietary available P levels (P < 0.05) (quadratic effect, P < 0.001). Signs of phosphorus deficiency were characterized by poor growth, slightly reduced mineralization and an increase in body lipid content. The blood chemistry analysis showed that dietary available P had distinct effects on enzyme activities of alkaline phosphatase, as well as contents of triacyglycerol and total cholesterol (P < 0.05). Broken-line analysis based on weight gain indicated the minimum available phosphorus requirement for the optimal growth of juvenile Chinese sucker was 7.4 g kg(-1). Based on the phosphorus content in whole body, vertebrae or scale indicated that the requirements were 8.3, 8.8 and 8.6 g kg(-1) respectively.
- 出版日期2011-4
- 单位华中农业大学
