Ultrasound induces contraction of the bladder smooth muscle

作者：Ren, Yan; Zhu, Yi; Liu, Li; Yu, Tinghe; Dong, Xiaojing^*

来源：International Urology and Nephrology, 2016, 48(8): 1229-1236.

DOI：10.1007/s11255-016-1298-8

• Postpartum urinary retention
• Low-intensity pulsed ultrasound
• Bladder smooth muscle
• L-type calcium channels

Purpose To investigate whether the treatment of overt postpartum urinary retention (PUR) with low-intensity pulsed ultrasound (LIPUS) was clinically effective and whether LIPUS could accelerate bladder smooth muscle (BSM) contraction by opening the L-type calcium channels and activating the Ca2+ signaling pathway. @@@ Methods Records of 136 patients undergoing PUR were retrospectively reviewed in two different groups for LIPUS and neostigmine between from 2014 to July 2015. The rats BSM strips in vitro were irradiated by LIPUS. The contraction frequency and amplitude were recorded with BL-410F biological experimental system. The BSM cells were constructed and identified by a-actin-specific antibody staining, and the intracellular Ca2+ concentration was analyzed by flow cytometry. @@@ Results The clinical trial indicated that LIPUS had potential therapeutic effect on PUR (80.6 vs. 64.1 %, p < 0.05), and the BSM strip contractility was increased by LIPUS (p < 0.001), and the concentration of Ca2+ was markedly enhanced by about twofold than that without LIPUS exposure (p < 0.01). Besides, nimodipine could suppress the contraction of BSM and the concentration of intracellular Ca2+ which was caused by ultrasound. @@@ Conclusions The results suggested LIPUS had potential therapeutic effect on PUR and the Ca2+ signaling pathway was involved in the mechanism. The ultrasound irradiation may provide a new method for PUR therapy.

• 出版日期2016-8
• 单位重庆医科大学