SNP genotyping by DNA photoligation: application to SNP detection of genes from food crops

作者：Yoshimura Yoshinaga^*; Ohtake Tomoko; Okada Hajime; Ami Takehiro; Tsukaguchi Tadashi; Fujimoto Kenzo

来源：Science and Technology of Advanced Materials, 2009, 10(3): 034603.

DOI：10.1088/1468-6996/10/3/034603

• biosensors
• DNA recognition
• ligation reactions
• photochemistry
• food crops

We describe a simple and inexpensive single-nucleotide polymorphism (SNP) typing method, using DNA photoligation with 5-carboxyvinyl-2'-deoxyuridine and two fluorophores. This SNP-typing method facilitates qualitative determination of genes from indica and japonica rice, and showed a high degree of single nucleotide specificity up to 10 000. This method can be used in the SNP typing of actual genomic DNA samples from food crops.

• 出版日期2009-6