Dihydroavenanthramide D protects pancreatic beta-cells from cytokine and streptozotocin toxicity

作者：Lv Na; Song Mi Young; Lee Young Rae; Choi Ha Na; Kwon Kang Beom; Park Jin Woo^*; Park Byung Hyun

来源：Biochemical and Biophysical Research Communications, 2009, 387(1): 97-102.

DOI：10.1016/j.bbrc.2009.06.133

• Dihydroavenanthramide D
• Cytokine
• Streptozotocin
• beta-Cells
• NF-kappa B

Dihydroavernanthramide D (DHAvD) is a synthetic analog to naturally occurring avenanthramide, which is the active component of oat. Although its anti-inflammatory, antiatherosclerotic, and antioxidant effects have been reported, the effect of DHAvD on type 1 diabetes is Unknown. Therefore, in this study, the effect of DHAvD on cytokine- or streptozotocin-induced beta-cell damage was investigated. Treatment of RINm5F insulinoma Cells or isolated islets with IL-1 beta and IFN-gamma induced beta-cell damage through a NF-kappa B-dependent signaling pathway. DHAvD-prctreated RINm5F cells or islets showed resistance to cytokine toxicity, namely suppressed nitric oxide (NO) production. reduced the inducible form of NO synthase expression, and decreased beta-cell destruction and the normal insulin secretion capacity. Furthermore, pretreatment with DHAvD blocked the development of type 1 diabetes in streptozotocin-treated mice. Prior injection with DHAvD maintained a normal range of plasma glucose and insulin, and retained immunoreactivity for insulin in the pancreas. These results suggest that DHAvD may be used to preserve functional beta-cell mass.

• 出版日期2009-9-11