Deletion of BCG Hip1 protease enhances dendritic cell and CD4 T cell responses

作者：Bizzell Erica; Sia Jonathan Kevin; Quezada Melanie; Enriquez Ana; Georgieva Maria; Rengarajan Jyothi^*

来源：Journal of Leukocyte Biology, 2018, 103(4): 739-748.

DOI：10.1002/JLB.4A0917-363RR

• immune evasion
• immunogenicity
• tuberculosis
• vaccine

Dendritic cells (DCs) play a key role in the generation of CD4 T cell responses to pathogens. Mycobacterium tuberculosis (Mtb) harbors immune evasion mechanisms that impair DC responses and prevent optimal CD4 T cell immunity. The vaccine strain Mycobacterium bovis Bacille Calmette-Guerin (BCG) shares many of the immune evasion proteins utilized by Mtb, but the role of these proteins in DC and T cell responses elicited by BCG is poorly understood. We previously reported that the Mtb serine protease, Hip1, promotes sub-optimal DC responses during infection. Here, we tested the hypothesis that BCG Hip1 modulates DC functions and prevents optimal antigen-specific CD4 T cell responses that limit the immunogenicity of BCG. We generated a strain of BCG lacking hip1 (BCGhip1) and show that it has superior capacity to induce DC maturation and cytokine production compared with the parental BCG. Furthermore, BCGhip1-infected DCs were more effective at driving the production of IFN- and IL-17 from antigen-specific CD4 T cells in vitro. Mucosal transfer of BCGhip1-infected DCs into mouse lungs induced robust CD4 T cell activation in vivo and generated antigen-specific polyfunctional CD4 T cell responses in the lungs. Importantly, BCGhip1-infected DCs enhanced control of pulmonary bacterial burden following Mtb aerosol challenge compared with the transfer of BCG-infected DCs. These results reveal that BCG employs Hip1 to impair DC activation, leading to attenuated lung CD4 T cell responses with limited capacity to control Mtb burden after challenge.

• 出版日期2018-4