Advanced glycation end products inhibit production and activity of matrix metalloproteinase-2 in human umbilical vein endothelial cells

作者：Gao L; Kang L; Chen Q; Chen C; Xu B^*; Jiang S

来源：Journal of International Medical Research, 2007, 35(5): 709-715.

• advanced glycation end products (AGEs)
• receptor for advanced glycation end products (RAGE)
• matrix metalloproteinase-2 (MMP-2)
• human umbilical vein endothelial cell (HUVECs)

The aim of this study was to investigate the effects of advanced glycation end products (AGEs) on the expression and activity of matrix metalloproteinases-2 (MMP-2) in human umbilical vein endothelial cells (HUVECs). Cultured HUVECs were incubated with various concentrations of AGEs-modified albumin or unmodified albumin for different time periods. Protein and gene expression of MMP-2 and the receptor for AGEs (RAGE) were measured by Western blot and reverse transcription-polymerase chain reaction, respectively. The activity of MMP-2 in the conditioned medium was measured by gelatin zymography. The AGE-modified albumin inhibited MMP-2 but increased RAGE protein and gene expression in HUVECs in a concentration- and time-dependent manner. An inhibition of MMP-2 activity was also detected in the conditioned medium of HUVECs incubated with AGEs-modified albumin. In conclusion, AGEs inhibited the expression and activity of MMP-2 in HUVECs; this may be mediated through upregulation of RAGE.

• 出版日期2007-10
• 单位南京大学; 中国人民解放军总医院