Abutilon mosaic geminivirus proteins expressed and phosphorylated in Escherichia coli

作者：Wege C; Jeske H^*

来源：Journal of Phytopathology, 1998, 146(11-12): 613-621.

DOI：10.1111/j.1439-0434.1998.tb04763.x

• geminivirus
• pET expression vector system
• tRNA supplementation
• antigens
• phosphorylation

In order to get more detailed information on single proteins involved in the life cycle of Abutilon mosaic geminivirus, special conditions were established allowing expression of the proteins encoded by different viral open reading frames (AC2, AC3, AV1, BC1, and BV1) in Escherichia coli by use of the pET expression vector system. These include cotransformation of a tRNA supplementation plasmid to overcome codon usage problems in the case of AC2. Protocols were developed to purify the virus proteins for production of antisera. Phosphorylation of AbMV proteins AC3, AV1, BC1, and BV1 in E. coli could be shown by autoradiographic detection of stably incorporated inorganic (PO43-)-P-32.

• 出版日期1998-12