A Proposal: Source of single strand DNA that elicits the SOS response

作者：Indiani Chiara^*; O' Donnell Mike

来源：Frontiers in Bioscience, 2013, 18(1): 312-323.

DOI：10.2741/4102

• SOS response
• Translesion Synthesis
• DNA repair
• lesion bypass
• DNA polymerase
• Sliding Clamp
• Review

Chromosome replication is performed by numerous proteins that function together as a %26quot;replisome%26quot;. The replisome machinery duplicates both strands of the parental DNA simultaneously. Upon DNA damage to the cell, replisome action produces single-strand DNA to which RecA binds, enabling its activity in cleaving the LexA repressor and thus inducing the SOS response. How single-strand DNA is produced by a replisome acting on damaged DNA is not clear. For many years it has been assumed the single-strand DNA is generated by the replicative helicase, which continues unwinding DNA even after DNA polymerase stalls at a template lesion. Recent studies indicate another source of the single-strand DNA, resulting from an inherently dynamic replisome that may hop over template lesions on both leading and lagging strands, thereby leaving single-strand gaps in the wake of the replication fork. These single-strand gaps are proposed to be the origin of the single-strand DNA that triggers the SOS response after DNA damage.

• 出版日期2013-1-1