Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging

作者：Chen Ya Wen; Pan Huay Ben; Tseng Hui Hwa; Chu Hsiao Chien; Hung Yu Ting; Yen Yi Chen; Chou Chen Pin^*

来源：International Journal of Molecular Sciences, 2013, 14(11): 21943-21959.

DOI：10.3390/ijms141121943

• diffusion-weighted magnetic resonance imaging
• epithelial
• mesenchymal
• apparent diffusion coefficient
• mouse xenografts

Epithelial-mesenchymal transition (EMT) is important for tumor metastasis. Detection of EMT protein expression and observation of morphological changes are commonly used to identify EMT. Diffusion-weighted magnetic resonance imaging (DW-MRI) and measuring apparent diffusion coefficient (ADC) values are noninvasive techniques for characterizing tumor microenvironments. We investigated the difference in ADC values between epithelial- and mesenchymal-like subcutaneous mouse xenografted tumors using DW-MRI. Epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 cells were generated from tumor suppressive Kruppel-like factor 4 (Klf4)-expressing mesenchymal-like MM189 and BL322 cells. The ADC values of xenografted tumors from epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 were significantly lower than those from their mesenchymal-like counterparts (p %26lt; 0.05 and p %26lt; 0.01, respectively). Our results suggested that DW-MRI is a potential tool for observing mesenchymal- or epithelial-like characteristics of subcutaneous xenografted tumors.

• 出版日期2013-11
• 单位中国医科大学