Limb remote ischemic preconditioning protects against cerebral ischemia through down-regulation of aquaporin-4

作者：Chen, Qiuyan; Wu, Fulin; Peng, Xiaolan; Jiang, Min; Chen, Tingting; Wei, Dingtai^*

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(7): 13878-+.

• Limb remote ischemic preconditioning
• MRI
• ischemia
• aquaporin-4

Objective: Limb remote ischemic preconditioning (LRP) represents a new strategy for stroke treatment, but its mechanism is still unclear. Here, we studied whether aquaporin-4(AQP4) was involved in the neuroprotection induced by LRP against ischemia. Method: Adult SD male rats were subjected to either LRP or sham surgery and then subsequently underwent 1 h middle cerebral artery occlusion (MCAo). Animals underwent MR T2FLAIR/DWI imaging 48 h after stroke and then neurological behavior evaluation and TTC staining were carried out. Western blot and immunofluorescence staining were used to investigate the expression of AQP4. Result: LRP improved the behavioral outcomes and reduced the infarct size. MRI results indicated that LRP ameliorated brain edema after cerebral ischemia. Western blot and immunostaining analysis showed that LRP down-regulated protein expression of AQP4. Conclusion: LRP executed protective effects against stroke and might ameliorate brain edema by inhibiting expression of AQP4.

• 出版日期2016
• 单位福建医科大学