Graphene Functionalized Graphite Electrode with Diphenylacetylene for Sensitive Electrochemical Determination of Adenosine-5'-triphosphate

作者：Ruan, Chengxiang^*; Xu, Li; Wang, Xiuzhen; Lou, Jin; Gao, Weimin; Sun, Wei

来源：Electroanalysis, 2012, 24(2): 286-292.

DOI：10.1002/elan.201100680

• Adenosine-5'-triphosphate
• Molecular wire
• Electrochemistry
• Graphene
• Diphenylacetylene

In this paper a molecular wire modified carbon paste electrode (MW-CPE) was firstly prepared by mixing graphite powder with diphenylacetylene (DPA). Then a graphene (GR) and chitosan (CTS) composite film was further modified on the surface of MW-CPE to receive the graphene functionalized electrode (CTS-GR/MW-CPE), which was used for the sensitive electrochemical detection of adenosine-5'-triphosphate (ATP). The CTS-GR/MW-CPE exhibited excellent electrochemical performance and the electrochemical behavior of ATP on the CTS-GR/MW-CPE was carefully studied by cyclic voltammetry with an irreversible oxidation peak appearing at 1.369 V (vs. SCE). The electrochemical parameters such as charge transfer coefficient (a) and electrode reaction standard rate constant (ks) were calculated with the results of 0.53 and 5.28 x 10(-6) s(-1), respectively. By using differential pulse voltammetry (DPV) as detection technique, the oxidation peak current showed good linear relationship with ATP concentration in the range from 1.0 nM to 700.0 mu M with a detection limit of 0.342 nM (3s). The common coexisting substances, such as uric acid, ascorbic acid and guanosine-5'-triphosphate (GTP), showed no interferences and the modified electrode was successfully applied to injection sample detection.

• 出版日期2012-2
• 单位江西科技师范大学; 青岛科技大学