Naturally Occurring Stable Isotopes Reflect Changes in Protein Turnover and Growth in Gilthead Sea Bream (Sparus aurata) Juveniles under Different Dietary Protein Levels

作者：Martin Perez Miguel^*; Fernandez Borras Jaume; Ibarz Antoni; Felip Olga; Fontanillas Ramon; Gutierrez Joaquim; Blasco Josefina

来源：Journal of Agricultural and Food Chemistry, 2013, 61(37): 8924-8933.

DOI：10.1021/jf402617h

• stable isotope fractionation
• muscle amino acid composition
• protein turnover
• dietary components
• optimal growth

Ideal nutritional conditions are crucial to sustainable aquaculture due to economic and environmental issues. Here we apply stable isotope analysis as an indicator of fish growth and feeding balance, to define the optimum diet for efficient growing conditions. Juveniles of gilthead sea bream were fed with six isoenergetic diets differing in protein to lipid proportion (from 41/26 to 57/20). As protein intake increased, delta N-15 and Delta delta N-15 of muscle and Delta delta N-15 and Delta delta C-13 of its protein fraction decreased, indicating lower protein turnover and higher protein deposition in muscle. This is reflected in the inverse relationship found between Delta delta N-15 and growth rate, although no differences were observed in either parameter beyond the protein/lipid proportion 47/23. Principal component analysis (PCA) also signaled 47/23 diet as the pivotal point with the highest growing efficiency, with isotopic parameters having the highest discrimination load. Thus, muscle isotope composition, especially N-15, can be used to evaluate nutritional status in farmed fish.

• 出版日期2013-9-18