摘要
In this paper, an ultrasensitive electrochemical immunoassay based on porous covalent organic framework materials and Au NPs-CoS/graphene oxide (GO) nanocomposite was developed for the detection of C-reactive protein (CRP). Au NPs-CoS/graphene oxide (GO) nanocomposite was synthesized, and employed as substrate because of their high conductivity to immobilize CRP antibody. A new porous covalent organic framework material (COF-LZU8) was functionalized by gold nanoparticles and doped with thionine (Thi) as the electron transfer mediator to obtain nanocomposite. The reduction peak signal of Thi/Au NPs-COF-LZU8 at -0.2 V could be directly detected by highly sensitive differential pulse voltammetry (DPV). Due to the high surface area of COF-LZU8, a great quantity of Thi could be adsorbed on the surface of COF, thus, the signal could be greatly amplified. Moreover, Thi/Au NPs-COF-LZU8 has strong adsorption capacity to the anti-CRP protein macromolecules due to the large specific surface area and good bio-affinity. The concentration of CRP antigen was proportional to the response current, so the quantitative detection of CRP was realized. Under the optimum conditions, the linear range of CRP was 0.05-150 ng/mL with the detection limit of 0.016 ng/mL (S/N = 3). This immunosensor provides a more effective and sensitive method for detection of CRP.
- 出版日期2018-4
- 单位云南师范大学