Astragalus Polysaccharide Suppresses the Expression of Adhesion Molecules through the Regulation of the p38 MAPK Signaling Pathway in Human Cardiac Microvascular Endothelial Cells after Ischemia-Reperfusion Injury

作者：Zhu Hai-Yan; Gao Yong-Hong; Wang Zhi-Yao; Xu Bing; Wu Ai-Ming; Xing Yan-Wei; Liu Bei; Lou Li-Xia; Chen Li-Xin^*

来源：Evidence-Based Complementary and Alternative Medicine, 2013, 2013: 280493.

DOI：10.1155/2013/280493

• NF-KAPPA-B
• P-SELECTIN
• KINASE
• ACTIVATION
• LEUKOCYTE
• CYTOKINES
• MODEL

Astragalus polysaccharide is a major component of radix astragali, a vital qi-reinforcing herb medicine with favorable immune-regulating effects. In a previous animal experiment, we demonstrated that astragalus polysaccharide effectively alleviates ischemia-reperfusion injury (IRI) of cardiac muscle through the regulation of the inflammatory reactions. However, the relationship between this herb and the cohesion molecules on the cell surface remains controversial. In this study, human cardiac microvascular endothelial cells (HCMECs) were used to validate the protective effects of astragalus under an IRI scheme simulated through hypoxia/reoxygenation in vitro. The results indicated that astragalus polysaccharide inhibited the cohesion between HCMECs and polymorphonuclear leukocyte (PMN) during IRI through the downregulation of p38 MAPK signaling and the reduction of cohesive molecule expression in HCMECs.

• 出版日期2013
• 单位北京中医药大学; 中国中医科学院; 中国中医科学院广安门医院; 中国中医科学院眼科医院