Fast and Efficient Molecule Detection in Localization-Based Super-Resolution Microscopy by Parallel Adaptive Histogram Equalization

作者：Li Yiming; Ishitsuka Yuji; Hedde Per Niklas; Nienhaus G Ulrich^*

来源：ACS Nano, 2013, 7(6): 5207-5214.

DOI：10.1021/nn4009388

• super-resolution microscopy
• localization microscopy
• molecule detection
• graphics processing unit (GPU)
• recall
• precision

In localization-based super-resolution microscopy, individual fluorescent markers are stochastically photoactivated and subsequently localized within a series of camera frames, yielding a final image with a resolution far beyond the diffraction limit. Yet, before localization can be performed, the subregions within the frames where the individual molecules are present have to be Identified-oftentimes in the presence of high background. In this work, we address the Importance of reliable molecule identification for the quality of the final reconstructed super-resolution image. We present a fast and robust algorithm (a-livePALM) that vastly improves the molecule detection efficiency while minimizing false assignments that can lead to Image artifacts.

• 出版日期2013-6